Lemonade, the multimillion-selling artist's new 'visual album', premiered on the US cable subscription channel HBO on Saturday night.
In between the album's 11 songs are several spoken elements, some of which contain words that many have interpreted as being about her relationship with her rapper husband.
In one song, Intuition, she says: "In the tradition of men in my blood, you come home at 3am and lie to me. What are you hiding?"
During stark visual imagery in another song, Formation, she is seen in water as she asks the question: "Are you cheating on me?"
It follows extensive speculation around the state of the power couple's marriage following reports Jay Z was accosted in a lift by Beyonce's sister Solange in 2014.
The three were forced to release a statement shortly after saying "families have problems and we're no different" and that "our family has worked through it," but the speculation has refused to go away.
According to Reuters, Beyonce makes clear in the last tracks of the new album that she has decided to reconcile with Jay Z and continue in her marriage to him.
Many music industry observers says the release shows Beyonce being typically innovative because of its visual quality and the way it was premiered.
Lemonade was shown for free on HBO, after being mysteriously trailed, following the pay-TV premiere of Jurassic World and before a major boxing match.